Table 4.

Ligation strength analysis of TgCPB and TgCPL for MHC class II molecules using SYFPEITHI

MHC II Allele1	Start-Stop2	Sequence3	Score4
TgCPB
H2-Ad	55–69	FSAEDVLNAFVSPES	31
H2-Ad	76–90	SIVAEQVVATSGNLT	27
H2-Ad	412–426	KDCEEQAYADNVHPF	27
H2-Ad	117–131	AGELLRLLLADSEDM	26
H2-Ak	43–57	LSEDTSVDPRESFSA	24
H2-Ak	72–86	SLFDSIVAEQVVATS	24
H2-Ak	149–163	IVRDSVLVSEKAFPS	24
H2-Ak	37–51	DDSLFPLSEDTSVDP	22
H2-Ed	313–327	NDRLCIRSQGKRLMP	28
H2-Ed	436–450	AYSLRSRDDVKRDMM	28
H2-Ed	229–243	RFRYLSLKDAKKLMG	26
H2-Ed	309–313	TEAFNDRLCIRSQGK	24
H2-Ek	86–100	SGNLTESAPRDRDSA	24
H2-Ek	313–327	NDRLCIRSQGKRLMP	22
H2-Ek	463–477	EDFLSYKSGVYKHVS	22
H2-Ek	37–51	DDSLFPLSEDTSVDP	20
HLA-DRB1*0101	4–18	RKSFRVLGTPLPFAA	35
HLA-DRB1*0101	13–27	PLPFAALAAILLLGC	34
HLA-DRB1*0101	470–484	SGVYKHVSGLPVGGH	33
HLA-DRB1*0101	71–85	ESLFDSIVAEQVVA T	32
TgCPL
H2-Ad	60–74	RAWIALVAAAVSLLV	31
H2-Ad	68–82	AAVSLLVFASFLIQW	30
H2-Ad	192–206	GVATELLNVLPSELP	30
H2-Ad	58–72	TARAWIALVAAAVSL	27
H2-Ak	18–32	GLENGELHQRRGVRA	22
H2-Ak	146–160	VYIHTHNQQGYSYSL	22
H2-Ak	196–210	ELLNVLPSELPAGVD	22
H2-Ak	256–270	ELMDCSRAEGNQSCS	20
H2-Ed	311–325	VKILGFKDVPRRSEA	30
H2-Ed	44–58	RTYFWKKFLRQRNFT	26
H2-Ed	170–184	RDEFRRKYLGFKKSR	26
H2-Ed	369–383	LVGYGTDKESKKDFW	26
H2-Ek	310–324	VVKILGFKDVPRRSE	24
H2-Ek	77–91	SFLIQWQGEDDRAVF	22
H2-Ek	365–379	HGVLLVGYGTDKESK	22
H2-Ek	6–20	THYVSFLNGEDDGLE	20
HLA-DRB1*0101	59–73	ARAWIALVAAAVSLL	34
HLA-DRB1*0101	60–74	RAWIALVAAAVSLLV	31
HLA-DRB1*0101	195–209	TELLNVLPSELPAGV	31
HLA-DRB1*0101	308–322	EKVVKILGFKDVPRR	30

¹ H2-Ad, H2-Ak, H2-Ed and H2-Ek alleles are mouse MHC class II molecules; the HLA-DRB1*0101 allele is a human MHC class II molecule.

² We chose 15 amino acids for analysis each time.

³ TgCPB or TgCPL sequences.

⁴ High score = high binding.