Figure 2. Probenecid-mediated growth inhibition is reversible.

HeLa cells were infected with C. trachomatis at an MOI = 1 and mock treated or treated with 2.0 mM probenecid immediately after infection. At 24 hr post infection, probenecid was washed away from one replicate and replaced with medium lacking drug (washout). All cultures were incubated an additional 24 hr and parallel cultures were fixed for immunofluorescence or disrupted for progeny counts. (A) Inclusions were stained with α-HSP60 and representative fields of view from each culture are shown. Bar = 5 µM. (B) Representative inclusion areas were computed and represented individually. A student’s T-test was performed to indicate statistical significance of differences (****; P<0.0001). (C) Data for progeny counts are represented as mean ± standard deviation of triplicate samples, and a student’s T-test was used to address significance (***; P<0.001).