Table 1.
qRT-PCR assay parameters: primer concentration, efficiency, linearity and dynamic range
| Genes | Primer concentration (nM) | Efficiency (%) | Error | Dynamic range (Cp) |
|---|---|---|---|---|
| TMBIM4 |
F600/R300 |
90.2 |
0.00291 |
23.90 – 32.39 |
| MMP-2 |
F600/R600 |
100.2 |
0.03450 |
25.57 – 32.68 |
| MMP-9 |
F300/R300 |
99.8 |
0.05740 |
30.50 – 36.28 |
| MT1-MMP |
F600/R600 |
100.1 |
0.02890 |
24.64 – 31.68 |
| TIMP-1 |
F600/R300 |
100.0 |
0.03110 |
25.24 – 31.25 |
| TIMP-2 |
F300/R300 |
103.4 |
0.02890 |
24.50 – 31.38 |
| RECK |
F600/R300 |
101.0 |
0.06310 |
27.05 – 33.78 |
| VEGF-A |
F300/R300 |
102.8 |
0.01020 |
26.56 – 32.49 |
| VEGF-164 | F300/R300 | 99.8 | 0.02690 | 28.54 – 35.19 |
F, forward primer; R, reverse primer.