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. 2013 May 20;8(5):e63621. doi: 10.1371/journal.pone.0063621

Table 1. Primer design.

SNP ID Primer Name Sequence TM(°C)
rs17277986 The PCR Primer rs17277986-L 5′-TCAGTTCTCCCATTTGTTGCT-3′ 59.73
[C/T] rs17277986-R 5′-AGGCTCTTGGAAGGCATTTT-3′ 60.21
The anchor probe rs17277986-SNP2 5′-ttttttttttCTCAGATTCCAAGAATTATTCAGC-3′ 57.14
rs6584777 The PCR Primer rs6584777-L 5′-CAGAGTGTGATCCCATCTCAA-3′ 58.70
[A/G] and rs6584777-R 5′-CTCCACCATGTGGAACTGTG-3′ 60.00
rs12251340 [G/T] The anchor probe rs6584777-SNP 5′-ttttttttttTAACTCCTGATATCCAAGTTTGTATTC-3′ 57.03
rs12251340-SNP 5′-CACCATGTGGAACTGTGAGT-3′ 55.17
rs10884402 The PCR Primer rs10884402-L 5′-TGCCTGTACAACGAGCTCAC-3′ 60.06
[A/G] rs10884402-R 5′-AGGTTCCCCTTTGCTGTTCT-3′ 60.11
The anchor probe rs10884402-SNP 5′-tttttttGCCAGCAGGAAAGAGAATGT-3′ 57.92
rs7078098 The PCR Primer rs7078098-L 5′-ACTCCTGATGCTCTGGGAGA-3′ 59.94
[C/T] rs7078098-R 5′-AGGGTGCTTCCAGATGTGAC-3′ 60.12
The anchor probe rs7078098-SNP 5′-tttttttttttttttttTGATGCTGATTAACAGTTTTCCC-3′ 58.97
rs950809 The PCR Primer rs950809-L 5′-CATTGACAGGCAAAGCAAGT-3′ 58.93
[C/T] rs950809-R 5′-GCATGGTTCTCTTTGGAGGA-3′ 60.20
The anchor probe rs950809-SNPr 5′-tttttttttttttttttttttTTGTAGGTGATGTTTGCAATCAGT-3′ 59.41
rs950809-SNP2 5′-ttttttttttttttttttttttttttttGATGAGGCATAGGGCTCACT-3′ 57.74