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. 2013 May 20;8(5):e63851. doi: 10.1371/journal.pone.0063851

Figure 1. Reduction of function in DAF-2 prevents recovery of pharyngeal pumping.

Figure 1

Young adult animals (15 per group) were grown overnight at the temperatures indicated (♦ 15°C; ▪ 20°C, or ▴ 25°C) and pharyngeal pumping was then evaluated on food (0 hr time point) and 2, 3.5, 6 and 24 hr after removal from bacteria. The data are expressed as the average pumps per minute (PPM). Standard deviations are represented by the error bars. Significant differences from the N2 controls are indicated by asterisks, *p<0.05; **p<0.01 as determined by ANOVA and a Newman-Keuls test. Results are shown for temperature-sensitive strains, A) daf-2(e1370) and B) daf-2(e1371).