Figure 5. Effect of mutations in genes encoding signaling molecules downstream of DAF-2 on the recovery response.

A) & B) The strains listed here were evaluated under standard conditions described above; gf and lf refer to gain-of-function and loss-of-function mutations, respectively. C) For these experiments, young adults (20 per group) from the N2 and daf-16; age-1 strains were incubated on plates with food and either DMSO (0.8%; vehicle control) or LY294002 (LY; 150 µM) for 1.5 hr at 15°C prior to food deprivation and evaluation of pharyngeal pumping. The 0-hr data point represents pumping on food in the presence of either DMSO or LY294002. The 99% confidence intervals for the control data are depicted for comparison.