Table 3. Inhibition of PI3Ks Affects Recovery of Pharyngeal Pumping^a.

Strain	Condition	On food	Off food	Off food	Off food	Off food
			2 hr	3.5 hr	6 hr	24 hr
				(PPM ± S.D.)
N2	DMSO	171.6±11.2	80.6±29.8	103.2±35.8	92.6±29.2	96±31.2
N2	LY	83.6±44.2**	45.4±26.4**	46.4±22.4**	45±38.6**	50.8±30.8**
daf-2(e1391) #	DMSO	181.1±10	9.0±20.8	4.4±12.8	7.6±22.4	8.2±26.4
daf-2(e1391) #	LY	103.8±47.2**	1.4±3.8	2.2±4.2	0.2±0.6	0.2±0.6
ins-1;ins-18	DMSO	131.2±20.4	62.4±21.8	59.8±27.2	55.4±25.6	50.8±30
ins-1;ins-18	LY	106.6±14.8**	28.2±24.6**	36.6±23.2*	44.4±23	44.8±27.8
unc-64(e246)	DMSO	104.2±14.4	53.2±28	27±16	31.8±21.2	23.2±16.6
unc-64(e246)	LY	85±19.6**	15.8±12**	17.2±11.6	19.8±11	9.4±10.8
nca1;nca-2	DMSO	162.8±12.4	70.6±29.6	78.6±27.6	73.8±31.2	65±30.1
nca1;nca-2	LY	99.8±36.2**	38.6±26.2**	53.8±32.4*	34±29.6**	45.4±27.4
arr-1(ok401)	DMSO	178.6±9.2	67.4±24.2	78.4±28.4	70.4±34.4	73.2±25.6
arr-1(ok401)	LY	68.4±36.2**	44.2±30.6	71±32	63±23.4	62.8±34.8
cca-1(ad1650)	DMSO	117.8±14.8	44.4±19.4	42.4±25.4	32.2±21	43.2±22.8
cca-1(ad1650)	LY	68.6±34.6**	22±15.4*	25.8±15.8	27.8±19.8	28.2±20.4
XA7401	DMSO	145.2±22.8	55.4±30.8	58.8±31.6	57.8±29.6	58.8±37.6
XA7401	LY	90±26.8**	38±27.6	42.2±30.4	44.8±35.2	54.2±36.8
pdk-1(mg142)	DMSO	177±16.8	85.8±40.6	105.2±40.2	105.4±33.2	118.2±38
pdk-1(mg142)	LY	89.8±60.2**	67.4±28.8	80.2±36.6	89.6±38.6	117.4±46

^aYoung adults (20 per group) were grown on plates treated with LY294002 (LY; 150 µM) or an equivalent amount of DMSO diluent for 1.5 hr before transfer off food. Pharyngeal pumping was measured periodically as before and the means and S.D. are summarized here. Significant differences from the corresponding DMSO control group have been indicated with asterisks:

^*p<0.05,

^**p<0.01.

^#The daf-2(e1391) strain was grown at 25°C overnight prior to testing, and during food deprivation.