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. 2013 May 20;8(5):e64026. doi: 10.1371/journal.pone.0064026

Figure 3. dsRNA-mediated in vivo knockdown of GST gene nlgst1-1 and reduction of GST activities in the dsRNA-fed BPHs.

Figure 3

BPHs were first fed on artificial diets containing 0.2, 0.4 and 0.8 µg/ml GFP-dsRNA or nlgst1-1 dsRNA for three days. Next, nlgst1-1 transcript levels in dsRNA pre-treated BPHs were monitored by quantitative real-time PCR (qRT-PCR) at 3 days post feeding. GST activities of dsRNA pre-treated BPHs were also determined by the spectrophotometric approach as described in Materials and Methods . All treatments were performed in triplicate, and the experiments were repeated three times. A. Reduction of nlgst1-1 transcript in BPHs fed on diet containing the target dsRNA. B. GST activities in BPHs fed on diets with various concentrations of target nlgst1-1 dsRNA. C. Correlation of GST activities with GST gene nlgst1-1 transcript levels in the dsRNA-fed BPHs. Data represent the means ± SD from three independent experiments. *, **and ***indicates significance at P<0.05, P<0.01 and P<0.001, respectively.