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. 2013 May 20;8(5):e64672. doi: 10.1371/journal.pone.0064672

Figure 6. Kinetic analysis of Grb2 recruitment to complexes containing EGFR and full length or truncated HER3.

Figure 6

HEK293FT cells were treated with 1 µM EGF or HRG. Cells expressing EGFR/Rluc8, Grb2/Venus and HER3 (a), cells expressing HER3/Rluc8, Grb2/Venus and EGFR (b), cells expressing EGFR/Rluc8, Grb2/Venus and truncated HER3 (HER3trunc; c), and cells expressing HER3trunc/Rluc8, Grb2/Venus and EGFR (d). BRET was measured in real-time and live cells before and after stimulation with the agonists as indicated. Data represent mean ± SEM of 4 independent experiments.