Figure 1. Chromatographic run order for MAF and LC-MS analysis.

Duplicate aliquots of CSF (denoted by ‘a’ and ‘b’) from each of six cognitively normal individuals (numbered 1 through 6) and fourteen aliquots of CSF (labeled P1 through P14) pooled from 100 individuals with mild [n = 9], very mild [n = 33], and no [n = 58] dementia of the Alzheimer type were selected for single block proteomic analysis. The order that samples underwent MAF (Y-axis) and then LC-MS analysis (X-axis) were independently randomized. A single sample (P7) that did not pass the 1D SDS-PAGE quality assessment (Fig. S1) and was not analyzed by LC-MS is not represented.