Figure 1.

Necrosis-Induced Sterile Inflammation Is Cell Type Specific

(A) IL-6 concentrations in conditioned media of macrophages (Macs), Jurkat cells, or VSMCs incubated with lysates from their respective necrotic cells or with IL-1α.

(B and C) Immunoblots of IL-1α content and processing in whole cell (WC) or necrotic lysates (NL) (B) or in necrotic cell lysates pretreated with protease inhibitors as indicated (C).

(D and E) IL-6 and MCP-1 concentrations in conditioned media of VSMCs incubated with necrotic VSMC lysates made in the presence of protease inhibitors (D), or with IL-1α alone, or with calpeptin or EGTA (E).

Data represent mean ± SD; ^∗p ≤ 0.007 versus control, n = 3; ^∗∗p ≤ 0.03 (MCP-1), p ≤ 0.002 (IL-6), n ≥ 4. NS, not significant. See also Figure S1.