Figure 3.

p33 IL-1α Shows Minimal Activity at Physiological Concentrations because of Lower Receptor Affinity

(A and B) Concentration-response curves to p17 and p33, minus control, in VSMCs (50 pM to 8 nM) (A) or EL4 cells (5 pM to 8 nM) (B).

(C and D) IL-2 concentrations in conditioned media from EL4 cells treated with p17 or p33 at 4 nM (C), and 0.1 nM p17 or 4 nM p33 (D), with increasing concentrations of IL-1RA.

Data represent mean ± SD; ^∗p ≤ 0.03, n = 3 (A), n ≥ 4 (B); for difference in IC₅₀ p < 0.0001 (C), p = 0.52 (D), n = 3. See also Figure S2.