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(a) Diagram of the position of the GFP tag at the N-terminus of PLY. (b) The lytic capacity of 0.05 μg/ml Atto488-labelled PLY and GFP-tagged PLY is equivalent to 0.1 μg/ml wild-type non-fluorescently labelled PLY in a propidium iodide permeabilisation live cell assay. This result is indicative of slightly improved pore formation by PLY in the presence of GFP and Atto488, most likely due to an improved monomer orientation.
