Input |
Match experimental intact masses to possible glycopeptides |
Employs biologically relevant protein sequences |
Provides biological filter for glycosite determination |
Incorporates allelic variation |
Does not require input of known glycans or peptide sequences |
Employs tandem MS/MS data |
Employs retention time |
Data handling |
Performs internal deconvoluting and deisotoping |
Compatible with multiple data input types |
Performs batch processing |
Retains biological context, such as position in protein sequence |
Output |
Solves peptide and glycan composition and glycosite for N- and O-linked glycopeptides |
Solves glycopeptide compositions in single protein, cocktail and complex biological samples |
Determines glycan structure |
Has low false positive and false negative rates |
Provides preliminary comparison of results from multiple samples |
Flexibility |
Provides options for different enzymatic cleavage types |
Open source |
Portability |
Works on Linux, Mac and Windows or web-interface |
Availability |
Freely available |