Table 3.
Results of in vivo 31P-NMR saturation transfer experiments performed on mouse skeletal muscle
| Variable | Group |
||||
|---|---|---|---|---|---|
| C | B | C+P | B+Pa | B+Pb | |
| n | 5 | 8 | 5 | 8 | 8 |
| Oxidative reaction: Pi → gATP | |||||
| ΔM/M0 | 0.24 ± 0.05 | 0.23 ± 0.05 | 0.15 ± 0.02 | 0.39 ± 0.07 | 0.31 ± 0.06 |
| T1obs (s) | 1.16 ± 0.1 | 1.33 ± 0.27 | 1.16 ± 0.4 | 1.33 ± 0.27 | 1.33 ± 0.27 |
| κf (s−1) | 0.21 ± 0.04 | 0.17 ± 0.04 | 0.14 ± 0.02 | 0.29 ± 0.05 | 0.24 ± 0.05 |
| ATP (μmol/g) | 1.21 ± 1.156 | 1.1 ± 0.0473 | 1.405 ± 0.346 | 1.18 ± 0.131 | 1.23 ± 0.158 |
| Pi (μmol/g) | 1.01 ± 0.28 | 1.41 ± .26 | 5.49 ± 0.28 | 1.41 ± 0.26 | 2.93 ± 0.56 |
| ATP synthesis rate (μmol/g/s) | 0.25 ± 0.09 | 0.06 ± 0.06 | 0.74 ± 0.09 | 0.36 ± 0.08 | 0.63 ± 0.11 |
| Anerobic reaction: PCr → gATP | |||||
| ΔM/M0 | 0.36 ± 0.018 | 0.19 ± 0.04 | 0.29 ± 0.04 | 0.24 ± 0.04 | 0.37 ± 0.04 |
| T1obs (s) | 1.16 ± 0.10 | 1.33 ± 0.27 | 1.16 ± 0.4 | 1.33 ± 0.27 | 1.33 ± 0.27 |
| κf (s−1) | 0.31 ± 0.016 | 0.08 ± 0.03 | 0.26 ± 0.04 | 0.18 ± 0.03 | 0.27 ± 0.04 |
| ATP (μmol/g) | 1.21 ± 0.156 | 1.1 ± 0.0473 | 1.405 ± 0.346 | 1.18 ± 0.131 | 1.23 ± 0.158 |
| PCr (μmol/g) | 2.28 ± 0.23 | 1.16 ± 0.232 | 3.76 ± 0.51 | 2.04 ± 0.09 | 3.25 ± 0.29 |
| ATP synthesis rate (μmol/g/s) | 0.72 ± 0.097 | 0.18 ± 0.065 | 0.92 ± 0.16 | 0.60 ± 0.11 | 0.87 ± 0.15 |
Values are means ± se; ΔM/M0 is the fractional change in Pi magnetization as a result of saturation transfer; T1obs is the observed spin lattice relaxation time of Pi during γATP saturation; κf is the rate constant for the reaction Pi → γATP, calculated as (1/T1obs) × (ΔM/M0). ATP synthesis is calculated as [Pi] × κf. B+P
a
B+P immediately after burn; B+P
b
B+P before and immediately after burn.