Figure 2.

Immunofluorescence analysis of unsorted and clonal BMSC populations. A–C) Nonclonally derived cultured BMSCs uniformly expressed Notch-2 (A), as confirmed by determining expression of the cytoskeleton protein vimentin (B) and coexpression of both (C). D–F) Expression of EphB2 was only seen in some of the nonclonally derived cultured BMSCs (D, F), indicating that high expression of EphB2 can be used to select a subpopulation of BMSCs. Constitutive expression of Vimentin by EphB2^−/low cells confirmed that lack of expression of EphB2 was not due to poor viability (E, F). DAPI (blue) labels all nuclei.