Figure 4.

EphB2^high BMSC and EGFP BMSCs express markers of ISCs after transplant and proliferate on engraftment. A–C) EGFP⁺ BMSCs after engraftment express Lgr5 (A) and Mushashi-1 (B) and continue to express EphB2 (C). D–F) EphB2^high BMSCs labeled with a lentiviral vector expressing EGFP⁺ cells engrafted in the crypt and villous area on transplantation (D); Ki67 labeling marks proliferating transplanted and endogenous cells (E); EGFP⁺ transplanted cells that are proliferating are marked with Ki67 labeling (F). Arrows indicate features enlarged in insets.