Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1972 Feb;49(2):249–251. doi: 10.1104/pp.49.2.249

The Problem of Reduced Nicotinamide Adenine Dinucleotide Oxidation in Glyoxysomes 1

J M Lord a, Harry Beevers a
PMCID: PMC365938  PMID: 16657934

Abstract

NADH is generated in glyoxysomes both in the glyoxylate cycle and in β-oxidation. No system has yet been described which would oxidize NADH in these organelles. A series of oxidants which might function by coupling NADH oxidation to O2 through endogenous carriers in the glyoxysomes was examined. Oxidation was brought about by ferricyanide or dichlorophenol-indophenol, but it was shown that this “diaphorase” activity is probably a contaminant. Hydroxypyruvate reductase (NAD-linked) is present in the glyoxysomes, and at very high substrate concentrations (>10 mm) this enzyme can also transfer electrons from NADH to glyoxylate. However, it is most unlikely that this concentration of glyoxylate is ever approached in glyoxysomes, where the malate synthetase would compete on much superior terms. The maximum rates of NADH oxidation observed in the presence of ferricyanide or glyoxylate are only a fraction of those required to reoxidize NADH at the rate occurring in vivo.

Full text

PDF
249

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Breidenbach R. W., Beevers H. Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm. Biochem Biophys Res Commun. 1967 May 25;27(4):462–469. doi: 10.1016/s0006-291x(67)80007-x. [DOI] [PubMed] [Google Scholar]
  2. Breidenbach R. W., Kahn A., Beevers H. Characterization of glyoxysomes from castor bean endosperm. Plant Physiol. 1968 May;43(5):705–713. doi: 10.1104/pp.43.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper T. G., Beevers H. Beta oxidation in glyoxysomes from castor bean endosperm. J Biol Chem. 1969 Jul 10;244(13):3514–3520. [PubMed] [Google Scholar]
  4. Cooper T. G., Beevers H. Mitochondria and glyoxysomes from castor bean endosperm. Enzyme constitutents and catalytic capacity. J Biol Chem. 1969 Jul 10;244(13):3507–3513. [PubMed] [Google Scholar]
  5. Feierabend J., Beevers H. Developmental studies on microbodies in wheat leaves : I. Conditions influencing enzyme development. Plant Physiol. 1972 Jan;49(1):28–32. doi: 10.1104/pp.49.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gerhardt B. P., Beevers H. Developmental studies on glyoxysomes in Ricinus endosperm. J Cell Biol. 1970 Jan;44(1):94–102. doi: 10.1083/jcb.44.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hutton D., Stumpf P. K. Fat Metabolism in Higher Plants. XXXVII. Characterization of the beta-Oxidation Systems From Maturing and Germinating Castor Bean Seeds. Plant Physiol. 1969 Apr;44(4):508–516. doi: 10.1104/pp.44.4.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KORNBERG H. L., BEEVERS H. The glyoxylate cycle as a stage in the conversion of fat to carbohydrate in castor beans. Biochim Biophys Acta. 1957 Dec;26(3):531–537. doi: 10.1016/0006-3002(57)90101-4. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Theimer R. R., Beevers H. Uricase and allantoinase in glyoxysomes. Plant Physiol. 1971 Feb;47(2):246–251. doi: 10.1104/pp.47.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tolbert N. E., Oeser A., Kisaki T., Hageman R. H., Yamazaki R. K. Peroxisomes from spinach leaves containing enzymes related to glycolate metabolism. J Biol Chem. 1968 Oct 10;243(19):5179–5184. [PubMed] [Google Scholar]
  12. Tolbert N. E., Yamazaki R. K., Oeser A. Localization and properties of hydroxypyruvate and glyoxylate reductases in spinach leaf particles. J Biol Chem. 1970 Oct 10;245(19):5129–5136. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES