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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: J Biol Chem. 2003 Nov 28;279(8):7064–7071. doi: 10.1074/jbc.M311794200

Fig. 2. Cos2 associates with vesicles and plasma membrane.

Fig. 2

A, total membranes prepared from S2 cells were washed in 0.15 m NaCl, then resuspended in 1.4 m sucrose, and overlaid with 1.22 m sucrose and then with 0.1 m sucrose. Following equilibrium density centrifugation (112,000 × g for 18 h), fractions were taken from the top of the tube. Each fraction was separated by SDS-PAGE and immunoblotted. Approximately 30% of Kinesin associates with vesicular membranes and is used here as a vesicular membrane marker (fractions 6–10) (61, 63). Fasciclin is a glycosylphosphatidylinositol-anchored protein and is used here as a plasma membrane marker (fractions 2–4) (66). The apparent accumulation of Fu in the plasma membrane to a greater degree than Cos2 is not consistently observed. B, S2 cells were transfected with HA-Cos2, then fixed, and stained with anti-HA-Alexa 488 and with 4,6-diamidino-2-phenylindole (not shown) to visualize DNA followed by confocal microscopy.

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