Skip to main content
. Author manuscript; available in PMC: 2014 May 10.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Apr 10;434(3):466–472. doi: 10.1016/j.bbrc.2013.03.097

Fig. 4. Knockdown of caveolin-1 restored fluid phase endocytosis in Prom2 expressing cells.

Fig. 4

HSFs were treated with Cav1 or negative siRNA for 5 days. (A) Immunoblot showed knockdown of Cav1 in cells treated with Cav1 siRNAs. Equal amounts of protein were loaded (actin immunoblot). (B) Cells treated with Cav1 or negative siRNA were transfected with Prom2-mKate for 48 h and then AF488-dextran uptake was studied as in Fig3. Prom2-transfected cells are outlined. Bar, 10 μm. (C) Quantitation of dextran uptake in Cav1 vs. negative siRNA-treated cells ± Prom2-mKate expression as in Fig3A. Prom2-transfected cells are outlined with dashed lines. Note the reduced level of dextran uptake in negative siRNA/Prom2-expressing cells compared to untransfected cells, but the lack of effect of Prom2 expression on dextran uptake in Cav1 siRNA-treated cells.