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Translation inhibition assay. Effect of cycloheximide (CHX) on HE-induced Cdk-1 and -2 mRNA expression. TIG-119 cells were pretreated for 2 h with 10 μg/mL CHX in serum-free medium and then treated with or without 0.75 mg/mL/mL of HE for 6 h. Total RNA was isolated and analysed for mRNA signals. Relative values of gene expression were depicted. Data represents the values of three independent experiments. Significant values were calculated by Student's t-test (P < 0.05).
