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. 2011 Sep;35(3):272–282. doi: 10.5142/jgr.2011.35.3.272

Table 1.

List of primers and PCR product size used for RT-PCR

Genes Primer sequence (5'-3') Size (b.p.)

Cyp11a1 Forward: ggt tca tcg acg ccgtct ac 648
Reverse: tac cgg aag tgc gtg gtg tt
RT1-Db1 Forward: gag tac acg cgc ttc gac ag 423
Reverse: gct cca cct ggc agg tat aa
Sv2b Forward: aac gcg gtg agc atc tca gc 1,074
Reverse: cca gcc gat cat cgc aga ct
β-actin Forward: ttg taa cca act ggg acg ata tgg 1,312
Reverse: ccg gac tcg tca tac tcc tg

PCR, polymerase chain reaction; RT, reverse-transcriptase; Cyp11a1, cytochrome P450, family 11, subfamily A, polypeptide 1; Sv2b, synaptic vesicle glycoprotein 2B.