Fig. 3. Determination of enzyme source on cyclooxygenase (COX)-1 and thromboxane A₂ synthase (TXAS) activity. (A) Detection of cytochrome c reductase activities in homogenates, microsomes, and cytosols of platelets. NADPH-cytochrome c reductase, the marker enzyme for microsomes in platelets, was assayed by using a NADHP cytochrome c reductase assay kit. One hundred micrograms of protein were used. (B) Western blot analysis of COX-1 and TXAS in homogenates, microsomes, and cytosols of platelets. The 30 μg (TXAS, COX-1) of total protein on homogenates, microsomes, and cytosols of platelets was separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the resolved proteins were transferred to polyvinylidene fluoride membranes and detected by enhanced chemiluminescence. H, homogenates; M, microsomes; C, cytosols. ^**p<0.001 compared with that of total saponin from Korean red ginseng-non-treated microsomes.