Table 1.
Time (min) | Flow (mL/min) | A%, ACN | B%, DW |
---|---|---|---|
| |||
Initial | 1.6 | 80 | 20 |
40 | 1.6 | 68 | 32 |
55 | 1.6 | 50 | 50 |
70 | 1.6 | 35 | 65 |
71 | 1.6 | 10 | 90 |
81 | 1.6 | 80 | 20 |
90 | 1.6 | 80 | 20 |
Column: Supelco Discovery C18, 5 μm, 250×4.6 mm; injection volume: 20 μL; column temperature: 40±2℃ ; detector: Waters 2998 photo diode array detector, 203±0.2 nm.
ACN, acetonitrile; DW, deionized water.