Fig. 2. (A) The changes of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, interleukin (IL)-1β, and IL-8 after sodium hydrogen sulfide (NaHS) alone or combined with Korean red ginseng extract (KRGE). NaHS significantly increased the expression of CSE. KRGE attenuated the expressions of CSE and CBS. Relative band intensity shows that Korean red ginseng has meaningful effect on attenuating the expression of CSE and CBS. The expressions of iNOS, COX-2, IL-8, and IL-6 were all increased with 100 μM NaHS, while KRGE significantly decreased these expressions of NaHS-induced inflammatory mediators. Relative band intensity showed that KRGE has meaningful effect on attenuating the expression of NaHS inducing inflammatory mediators. (B) The changes of angiogenic factors including hypoxia inducible factor (HIF)-1α, IL-6, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF). 100 μM NaHS increased these angiogenic factors significantly, while KRGE significantly decreased these expressions of NaHS-induced angiogenic factors. (C) Reverse transcription polymerase chain reaction for matrix metalloproteinases (MMPs). NaHS significantly increased the expressions of MMP-2, MMP-3, and MMP-9, while KRGE could attenuate these expressions of H₂S-driven MMPs. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.