Table 1.
Gene name | Sequence |
---|---|
| |
IL-1β | Forward 5’-ATA AGC CCA CTC TAC AGC T-3’ |
Reverse 5’-ATT GGC CCT GAA AGG AGA GA-3’ | |
IL-6 | Forward 5’-AGC GCC TTC GGT CCA GTT GC-3’ |
Reverse 5’- CCG AAG AGC CCT CAG GCT GGA-3’ | |
IL-8 | Forward 5′-TGG GTG CAG AGG GTT GTG-3′ |
Reverse 5′-CAG ACT AGG GTT GCC AGA TTT A-3′ | |
HIF-1α | Forward 5’-ACA GCA GCC AGA CGA TCA TGC-3’ |
Reverse 5’-AAA TGA GCT GTC TGT GAT CCA GCA TT-3’ | |
VEGF | Forward 5′-TCG GGC CTC CGA AAC CAT G-3′ |
Reverse 5′-GGT TCC CGA AAC CCT GAG G-3′ | |
MMP-2 | Forward 5-’GAG TAA GGG GAT CGC CGT GCA-3’ |
Reverse 5’-AAG AGG TTG CAA CTC TCC TTG G-3’ | |
MMP-3 | Forward 5’-TGC AGA AGT TCC TTG GAT TGG AG-3’ |
Reverse 5’- GGA GAA TGT GAG TGG AGT CAC C-3’ | |
MMP-9 | Forward 5’-GCG GAC ATT GGG AAC CAG CTG TA-3’ |
Reverse 5’-GAC GCG CCT GTG TAC ACC CAC A-3’ | |
PDGF | Forward 5’-AGG AAG CCA TTC CCG CAG TT-3’ |
Reverse 5’-CTA ACC TCA CCT GGA CCT CT-3’ | |
bFGF | Forward 5’- TAT GAA GGA AGA TGG ACG GC-3’ |
Reverse 5’-AAC AGT ATG GCC TTC TGT CC-3’ | |
iNOS | Forward 5′-GGC CTC TCA GCT CAC CCC GA-3′ |
Reverse 5′-CCA GGC GCA CTG TCT GGT GG-3 | |
COX-2 | Forward 5′-GGT CTG GTG CCT GGT CTG ATG ATG-3′ |
Reverse 5′-GTC CTT TCA AGG AGA ATG GTG C-3′ | |
GAPDH | Forward 5′-AGG TCG GAG TCA ACG GAT TTG G-3′ |
Reverse 5′-ACA GTC TTC TGG GTG GCA GTG ATG-3′ | |
CBS | Forward 5’-TGG ATG CAG GAT CAT TGG GGT G-3’ |
Reverse 5’-TCC CGG AGG ATC TCG ATG GTG-3’ | |
CSE | Forward 5’-AAG AAC CTA AAF CTA TTT ACT CTG-3’ |
Reverse 5’-TTG GTC CAT TTA ATT ACT CAG GAA-3’ | |
HO-1 | Forward 5’-AGA CGG CTT CAA GCT GGT GA-3’ |
Reverse 5’-TAG GGG ATG ACC TCC TGC CA-3’ |
IL, interleukin; HIF, hypoxia inducible factor; VEGF, vascular endothelial growth factor; MMP, matrix metalloproteinase; PDGF, platelet-derived growth factor; bFGF, basic fibroblast growth factor; iNOS, inducible nitric oxide synthase; COX, cyclooxygenase; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; CBS, cystathionine β-synthase; CSE, cystathionine γ-lyase; HO, heme oxygeanse.