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. 2012 Apr;36(2):135–145. doi: 10.5142/jgr.2012.36.2.135

Table 1.

Primer sequence for polymerase chain reaction

Gene name Sequence

IL-1β Forward 5’-ATA AGC CCA CTC TAC AGC T-3’
Reverse 5’-ATT GGC CCT GAA AGG AGA GA-3’
IL-6 Forward 5’-AGC GCC TTC GGT CCA GTT GC-3’
Reverse 5’- CCG AAG AGC CCT CAG GCT GGA-3’
IL-8 Forward 5′-TGG GTG CAG AGG GTT GTG-3′
Reverse 5′-CAG ACT AGG GTT GCC AGA TTT A-3′
HIF-1α Forward 5’-ACA GCA GCC AGA CGA TCA TGC-3’
Reverse 5’-AAA TGA GCT GTC TGT GAT CCA GCA TT-3’
VEGF Forward 5′-TCG GGC CTC CGA AAC CAT G-3′
Reverse 5′-GGT TCC CGA AAC CCT GAG G-3′
MMP-2 Forward 5-’GAG TAA GGG GAT CGC CGT GCA-3’
Reverse 5’-AAG AGG TTG CAA CTC TCC TTG G-3’
MMP-3 Forward 5’-TGC AGA AGT TCC TTG GAT TGG AG-3’
Reverse 5’- GGA GAA TGT GAG TGG AGT CAC C-3’
MMP-9 Forward 5’-GCG GAC ATT GGG AAC CAG CTG TA-3’
Reverse 5’-GAC GCG CCT GTG TAC ACC CAC A-3’
PDGF Forward 5’-AGG AAG CCA TTC CCG CAG TT-3’
Reverse 5’-CTA ACC TCA CCT GGA CCT CT-3’
bFGF Forward 5’- TAT GAA GGA AGA TGG ACG GC-3’
Reverse 5’-AAC AGT ATG GCC TTC TGT CC-3’
iNOS Forward 5′-GGC CTC TCA GCT CAC CCC GA-3′
Reverse 5′-CCA GGC GCA CTG TCT GGT GG-3
COX-2 Forward 5′-GGT CTG GTG CCT GGT CTG ATG ATG-3′
Reverse 5′-GTC CTT TCA AGG AGA ATG GTG C-3′
GAPDH Forward 5′-AGG TCG GAG TCA ACG GAT TTG G-3′
Reverse 5′-ACA GTC TTC TGG GTG GCA GTG ATG-3′
CBS Forward 5’-TGG ATG CAG GAT CAT TGG GGT G-3’
Reverse 5’-TCC CGG AGG ATC TCG ATG GTG-3’
CSE Forward 5’-AAG AAC CTA AAF CTA TTT ACT CTG-3’
Reverse 5’-TTG GTC CAT TTA ATT ACT CAG GAA-3’
HO-1 Forward 5’-AGA CGG CTT CAA GCT GGT GA-3’
Reverse 5’-TAG GGG ATG ACC TCC TGC CA-3’

IL, interleukin; HIF, hypoxia inducible factor; VEGF, vascular endothelial growth factor; MMP, matrix metalloproteinase; PDGF, platelet-derived growth factor; bFGF, basic fibroblast growth factor; iNOS, inducible nitric oxide synthase; COX, cyclooxygenase; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; CBS, cystathionine β-synthase; CSE, cystathionine γ-lyase; HO, heme oxygeanse.