Fig. 3. Effect of sun ginseng (SG) on lipid peroxiation against tertbutyl hydroperoxide (t-BHP)-induced-oxidative stress in HepG2 cells. Cells were pre-treated with the indicated concentration of SG (5, 10, and 20 μg/mL) and then exposed to 2.5 mM of t-BHP for 3 h as mentioned. After incubation, culture medium was precipitated with 50% trichloroacetic acid and thiobarbituric acid reactive substance (TBARS) assay was performed. The lipid peroxidation levels were showed by malondialdehyde concentration made by the hydrolysis of 1,3,3-tetraethoxypropane. Data represent the mean±SD of triplicate experiments. Differences were considered significant at ^##p<0.01 vs. negative control and ^*p<0.05 and ^**p<0.01 vs. t-BHP-treated cells.