Table 1.

Ginsenosides identified from raw and multi-steamed Panax quinquefolius

Peak no.	tR (min)	Identity	Molecular formula	[M-H]― (m/z)	[M-H+HCOOH]― (m/z)	Fragmentions of [M-H]― (m/z)	References
1	2.62	Rg1	C42H72O14	799.48	845.49	637.43 [M−H-(Glc-H2O)]−; 475.38 Agl	[18,37]
2	2.64	Re	C48H82O18	945.54	991.55	799.48 [M−H-(Rha-H2O)]− ; 783.49 [M−H-(Glc-H2O)]− ;	[10,18,37]
						637.43 [M−H-(Rha-H2O)-(Glc-H2O)]− ; 475.38 Agl
3	4.82	24(S)-Pseudo F11	C42H72O14	799.49	845.49	653.43 [M−Rha-H2O]−; 635.41 [M−H-(Rha-H2O)-H2O]−;	[10,37]
						491.37 [M−H-(Rha-H2O)-(Glc-H2O)]−
4	7.52	Rb1	C54H92O23	1107.59	1153.59	945.54 [M−H-(Glc-H2O)]− ; 783.49 [M−H-2(Glc-H2O)]− ;	[18,37]
						621.43 [M−H-3(Glc-H2O)]−;
5	7.96	Ma-Rb1	C57H94O26	1193.60	—	1149.61 [M−H-CO2]−	[18,30,37]
6	8.76	Rc	C53H90O22	1077.59	1123.59	945.54 [M−H-(Ara(f)-H2O)]−; 621.44 [M−H-(Ara(f)-H2O-2(Glc-H2O)]−;	[10,18,37]
						783.48 [M−H-(Ara(f)-H2O-(Glc-H2O)]−; 915.53 [M−H-(Glc-H2O)]−
7	9.48	Ma-Rc	C56H92O25	1163.58	—	1119.60 [M−H-CO2]−	[18,30,37]
8	10.24	Rb2	C53H90O22	1077.59	1123.59	945.54 [M−H-(Ara(f)-H2O)]−; 915.53 [M−H-(Glc-H2O)]−;	[16,18,37]
						783.48 [M−H-(Ara(f)-H2O-(Glc-H2O)]−;
9	10.98	Ma-Rb2	C56H92O25	1163.58	—	1119.60 [M−H-CO2]−	[18,30,37]
10	11.98	Rd	C48H82O18	945.55	991.56	783.49 [M−H-(Glc-H2O)]−; 621.43 [M−H-2(Glc-H2O]−	[18,30,37]
11	12.24	Ma-Rd	C51H84O21	1031.54	—	987.56 [M−H-CO2]−	[18,30,37]
12	6.61	20(S)-Rg2	C42H72O13	783.48	829.50	621.44 [M−H-(Glc-H2O)]−; 459.38 [M−H-2(Glc-H2O)]−	[16,18,37]
13	6.94	20(S)-Rh1	C36H62O9	637.43	683.44	475.38 Agl	[18,37]
14	14.98	20(S)-Rg3	C42H72O13	783.49	829.50	621.44 [M−H-(Glc-H2O)]−; 459.38 [M−H-2(Glc-H2O)]−	[16,18,37]
15	15.18	20(R)-Rg3	C42H72O13	783.49	829.50	621.44 [M−H-(Glc-H2O)]−; 459.38 [M−H-2(Glc-H2O)]−	[16,18,37]
16	16.66	Rk1	C42H70O12	765.48	811.49	603.43 [M−H-(Glc-H2O)]−	[16,18,30,37]
17	16.84	Rg5	C42H70O12	765.48	811.48	603.43 [M−H-(Glc-H2O)]−	[16,18,37]
18	7.18	20(R)-Rh1	C36H62O9	637.43	683.44	475.38 Agl	[36,42]
19	17.92	Rh2	C36H62O8	621.44	667.44	459.38 Agl	[16,18,37]
20	13.42	F4	C42H70O12	765.48	811.49	619.43 [M−H-(Rha-H2O)]−	[16,18,37]
21	13.72	Rk3	C36H60O8	619.42	665.42	457.36 [M−H-(Glc-H2O)]−	[16,18,37]
22	14.12	Rh4	C36H60O8	619.42	665.43	457.36 [M−H-(Glc-H2O)]−	[16,18,37]
23	13.18	Rg6	C42H70O12	765.48	811.49	619.43 [M−H-(Rha-H2O)]−	[16,18,37]
24	15.64	20(S)-Rs3	C44H74O14	825.50	871.51	783.49 [M−H-Ac]− ; 621.44 [M−H-Ac-(Glc-H2O)]−	[16,18,37]
25	15.82	20(R)-Rs3	C44H74O14	825.50	871.51	783.49 [M−H-Ac]− ; 621.44 [M−H-Ac-(Glc-H2O)]−	[16,18,37]
26	17.12	Rs5	C44H72O13	807.49	—	765.48 [M−H-Ac]−; 603.42 [M−H-Ac-(Glc-H2O)]−;	[16,18,30,37]
27	17.36	Rs4	C44H72O13	807.49	—	765.48 [M−H-Ac]−; 603.42 [M−H-Ac-(Glc-H2O)]−;	[16,18,37]
28	18.48	Rk2	C36H60O7	603.40	649.42	441.34 [M−H-(Glc-H2O)]−	[43]
29	18.78	Rh3	C36H60O7	603.40	649.42	441.34 [M−H-(Glc-H2O)]−	[43]
30	2.32	20(S)-Rf2	C42H74O14	801.50	847.51	655.44 [M−H-(Rha-H2O)]−; 637.43 [M−H-(Rha-H2O)-H2O]−;	[37,41]
						493.39 [M−H-(Rha-H2O)-(Glc-H2O)] −
31	7.36	20(R)-Rg2	C42H72O13	783.48	829.50	621.44 [M−H-(Glc-H2O)]−; 459.38 [M−H-2(Glc-H2O)]−	[18,37]
32	13.28	F2	C42H72O13	783.49	829.50	621.44 [M−H-(Glc-H2O)]−	[10,37]