Table 1.
Polymorphic EST-SSR markers developed in this study
| Marker | Original sequence ID | SSR motif | Primer sequence | Ta (℃) | Estimated size | Putative gene function | |
|---|---|---|---|---|---|---|---|
|
| |||||||
| gm99 | CN846560.1 | (TA)32 | F | AATAACCTGATTTTAAACGGTGACA | 52 | 277 | Beta glucosidase 15 |
| R | GACTATTGTAAGCGTTATCCCAAGA | ||||||
| gm104 | CN847863.1 | (GAA)7 | F | TGAATCTGGATAGATACACGACAGA | 52 | 187 | No hits |
| R | TGAAGTGTCGACAGAAGTGGTATAA | ||||||
| gm105 | DV553852.1 | (TTA)7 | F | GGTTCTTGGAGAAGAGAAAGGATAG | 52 | 288 | Jasmonate ZIM-domain protein |
| R | GATATACTTGACGGCTCTGAGAAAA | ||||||
| F | CGAAAGTGACTAACACCACTTTTCT | ||||||
| gm119 | pgcn1164 | (TA)17 | R | TGTAGTGAAACTGTGAAAGCAAGAG | 54 | 158 | Abscisic acid receptor PYL4 |
| F | CCATCCTAGCGGTCTAGTAAAGACT | ||||||
| gm123 | pgcn191 | (TA)18 | R | CACTCAGCTCAATGATATCAGCA | 52 | 169 | No hits |
| F | AAGCAAGTTGATGGAATATATGAGC | ||||||
| gm129 | pgcn2845 | (CTA)6 | R | CAGTTTTCTCTTCCTGGATATCGTA | 56 | 97 | Transcription factor WRKY4 |
| F | TTGATGAAGAAACAGACATGAAAGT | ||||||
| gm131 | pgcn2983 | (TA)10 | R | TACGTGCATTTATTTTGGCATTATT | 56 | 150 | Beta-amylase |
| F | GCCTCTTCTAGAGATCTGTCATTTG | ||||||
| gm132 | pgcn3023 | (ATTTG)6 | R | GAGATAAAAATACAGCTCCGTGACT | 56 | 133 | No hits |
| GGAGCATGACTGTCAAATCTACATA | |||||||
| gm134 | pgcn3339 | (CAG)7 | R | CTCCCGGAAAAGAGACTTAATTTAC | 56 | 130 | ELF4-like protein |
| F | ATCCCTAAGAGAAACCCAGATCTAA | ||||||
| gm137 | pgcn3646 | (TTTC)7 | R | ACACCAATCAATTTCACTTCTTGAT | 56 | 122 | Glutathione S-transferase |
| F | GTAAACTCGACCAGACAAAACCTTA | ||||||
| gm139 | pgcn3803 | (TA)10 | R | CTCATTTTTAGGCAATGGATTCTAC | 56 | 105 | No hits |
| F | GACAGAACTTCTTCATCTGCAATTT | ||||||
| gm148 | pgcn4474 | (TA)10 | R | GGCTCTCTCTTGTACCATTTAAACA | 56 | 145 | Basic blue copper protein |
| F | TTGAAGCTTACATCCTAGCAAATTA | ||||||
| gm159 | pgcn732 | (TA)7 | R | ATATAACAACAAAGCCAAGAAGCAC | 54 | 150 | PR10-2 |
| F | AGAAAACCAAATAAAGCCACACAC | ||||||
| gm160 | pgcn733 | (TA)21 | R | CACCGATAAGTCAATCCAACTATTC | 54 | 145 | Pathogenesis-related protein 10 |
| F | TTTTACTCATCTCCGTTTACACACA | ||||||
| gm166 | pgDC01027F02 | (TA)11 | R | AATTATCAGCACCCACAATTAAAAA | 54 | 123 | MADS-box protein |
| F | TCTTTAGAAAAATCTATTAGGGGTC | ||||||
| gm169 | pgDC03005G03 | (TA)10 | R | AGTAGGATCAAAATAATTACGAGGT | 56 | 194 | No hits |
| F | CCTCCAACATTATTTCAGTCTCAGT | ||||||
| gm175 | pgDC05024D08 | (TCC)9 | R | TAGTGGTAGCAGCTTAGGAGGAGTA | 56 | 123 | Hypothetical protein |
| F | CCTTGCTGCTCAAATTAATCTAAAA | ||||||
| gm176 | pgDC06005G10 | (TA)11 | R | CTCACTACTACTTGCCGCTTCTTC | 56 | 148 | Mini zinc fi nger 1 |
| F | TGGTTGACAAAGAAATTAACCAAAT | ||||||
| gm184 | pgPG04002D08 | (TTC)6 | R | GACCAAAAAGATCCGTCGTAAAG | 56 | 133 | Unnamed protein product |
EST, expressed sequence tag; SSR, simple sequence repeat.