Table 1.
Effects of metal ions and chemical agents on the activity of purified BglF3
| Metal ions or reagents | Relative activity±SD (%) | |
|---|---|---|
|
| ||
| 1 mM | 10 mM | |
|
| ||
| NaCl | 143.9±1.6 | 108.2±1.5 |
| KCl | 128.2±3.5 | 110.2±1.6 |
| MgCl2 | 152.6±3.3 | 112.5±3.4 |
| MnCl2 | 53.3±1.0 | 39.7±2.4 |
| CoCl2 | 58.8±3.5 | 44.3±2.7 |
| ZnCl2 | 4.7±1.7 | 2.9±2.5 |
| CaCl2 | 92.5±2.6 | 81.0±1.9 |
| CuCl2 | 6.3±1.4 | 6.0±2.0 |
| HgCl2 | 0 | 0 |
| SDS | 39.9±3.2 | 1.98±1.7 |
| EDTA | 76.4±2.7 | 68.9±3.7 |
| β-mercaptoethanol | 93.8±1.7 | 91.9±1.8 |
| DTT | 96.0±4.1 | 97.9±3.4 |
| Control | 100.0±2.6 | 100.0±1.5 |
SDS, sodium dodecyl-sulfate; EDTA, ethylenediaminetetraacetic acid; DTT, dithiothreitol.