Fig. 2. Sequence homology and phylogenetic analysis of PgGST with other glutathione S-transferase (GST) proteins. (A) comparison of the putative amino acids sequence of PgGST with those of GST genes of theta class from other species with GenBank accession no. in parenthesis; Glycine max (AAG34813), Arabidopsis thaliana (AAM98138), Euphorbia esula (AAF64449), Oryza sativa (AAK98534), Canis familiaris (XP534751), Homo sapiens (AAV38754), Bos taurus (AAI11290), Mus musculus (NP598755), Rattus norvegicus (NP445745), Danio rerio (AAH56725), Anopheles gambiae (AAM61892), and Aedes aegypti (AAV68399). Hyphen was inserted within amino acid sequence to denote gap. Shadow box means well conserved residues, * represents conserved amino acid and, : represents very similar amino acid. Glutathione-binding residues are indicated by red boxes. (B) Phylogeny of the theta class GST protein family from Panax ginseng and other species. Phylogenetic analysis is based on the deduced amino acid sequences of GST genes from various species. Neighbor-joining method was used and the branch lengths are proportional to divergence, with the scale of 0.1 representing 10% changes.