Fig. 5. Confirmation of transgenes in tobacco by polymerase chain reaction (PCR) and southern blot analysis. (A) PCR analysis of 35S cauliflower mosaic virus promoter (CaMV), glutathione S-transferase of Panax ginseng (PgGST), neomycin phosphotransferase II (NPTII), nopaline synthase terminator (NOS), and internal transcribed spacer (ITS) gene as control from non-transgenic (N) and two transgenic tobacco lines (T1 and T2) was conducted. (B) Southern blot hybridization of DNA prepared from transgenic tobacco plants. DNA (20 μg) was cut by restriction enzymes EcoRΙ or HindⅢ, separated on 1.3% agarose gel, transferred to a membrane and hybridized to a DIG-labeled PgGST-specific probe. (C) reverse transcriptase-PCR and (D) glutathione S-transferase (GST) activity of non-transgenic (N) and two transgenic lines (T1 and T2).
