Fig. 4. Effects of total saponin from Korean red ginseng (TSKRG) on adenosine triphosphate (ATP) release in resting and thrombin-stimulated platelets and light transmission (%) coincidentally. (A) Effects of TSKRG on ATP release in resting and thrombin-stimulated platelets. (B) Light transmission (%) with ATP release on thrombin-stimulated platelets. a, thrombin; b, thrombin+TSKRG (25 μg/mL); c, thrombin+TSKRG (50 μg/mL); d, thrombin+TSKRG (100 μg/mL); and e, thrombin+TSKRG (150 μg/mL). Washed platelets (10⁸/mL) were preincubated with or without various concentrations of TSKRG for 3 min in the presence of 2 mM CaCl₂ and then stimulated with thrombin (0.5 U/mL) for 5 min at 37℃. The reaction was terminated by the addition of ice-cold 2mM EDTA, and centrifuged and supernatant were used for the assay of ATP release. ATP release was measured in a luminometer using an ATP assay kit. Data are expressed as means±SEM (n=4). **p<0.001.