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. 2012 Sep 27;1(10):719–724. doi: 10.5966/sctm.2012-0056

Figure 1.

Figure 1.

Isolation of surface epithelium separate from SMG cells. (A): Alcian blue/periodic acid-Schiff (AB/PAS) staining showed the SMGs and their ducts opening onto the surface epithelium. (B): Immunofluorescent staining of tracheal sections showed that CD166 stained all surface epithelial cells and SMG duct cells but not SMG tubules, whereas CD44 (C) and NGFR ([D], merged in [E]) stained only basal cells of surface epithelium and were absent in SMG tubules. (F): AB/PAS staining of tracheas after 2 hours of dispase digestion showed complete detachment of surface epithelium with almost complete preservation of SMGs and ducts. (G): Higher magnification of the inset in (F). Scale bars = 50 μm. Abbreviations: NGFR, nerve growth factor receptor; αSMA, α-smooth muscle actin; SMG, submucosal gland.