Figure 7.

Prevention of brain tumor formation by stem-like glioma-initiating cells implanted in the brain parenchyma via transient, systemic administration of metformin. (A–D): Nude mice implanted intracranially with SJ28P3 (A, B) or #38 (C, D) cells (1 × 10⁴) underwent transient, systemic administration (intraperitoneal delivery, once daily for 5 or 10 consecutive days) of metformin (500 mg/kg per day) or vehicle control, which started on the next day of intracranial implantation. (A, C): Representative hematoxylin and eosin staining of brain sections from mice sacrificed at 25 days after implantation. (B, D): Survival of mice was evaluated by Kaplan-Meier analysis. Scale bars = 2 mm (A, C). *, p < .05; **, p < .01.