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. 2012 Oct 23;1(11):771–782. doi: 10.5966/sctm.2010-0031

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©AlphaMed Press 1066-5099/2012/$20.00/0

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Figure 2. — Differentiation potential of mesenchymal stem cells in long-term culture. Osteogenic and adipogenic differentiation was induced for 4 and 14 days after every second passage of cultivation. Expression of osteogenic markers Runx-2, ALP, Col-1, and OSC (A–D) and adipogenic markers PPAR-γ, CEBP-α, FAB-4, and LPL (E–H) mRNA was analyzed by quantitative reverse transcription-polymerase chain reaction. (Mean ± SEM of three experiments of duplicates.) *, p < .05, **, p < .01, ***, p < .001 compared with P4. Abbreviations: ALP, alkaline phosphatase; CEBPα, Ccaat enhancer binding protein-α; Col-1, collagen 1; OSC, osteocalcin; FAB-4, fatty acid binding protein 4; LPL, lipoprotein lipase; P, passage; PPAR, peroxisome proliferator-activated receptor.