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. 2012 Apr 10;1(4):298–308. doi: 10.5966/sctm.2011-0045

Figure 2.

Figure 2.

Expression of differentiation neural markers by normal (hNSC), v-myc-immortalized (IhNSC), and human glioblastoma (hGBM)-derived stem-like cells. hNSCs, IhNSCs, and hGBM-derived stem-like cells (indicated as BCSCs) were differentiated onto an adhesive substrate in the absence of mitogenic factors for 10 days. (A–C): Immunostaining showing the definite segregation of the early neuronal marker βtubIII (red) and the astroglial marker GFAP in hNSCs (A) and IhNSCs (B) and, conversely, a partial colocalization of the two markers in BCSCs (arrows in [C]). (D–I): Immunostaining of hNSCs, IhNSCs, and BCSCs with antibodies recognizing late neuronal marker MAP2 (D–F) and oligodendrocyte marker GalC (G–I). Total nuclei are shown by Dapi staining (blue). Scale bars = 50 μm. Abbreviations: βtubIII, β-tubulin III; BCSC, brain cancer stem cell; Dapi, 4′,6-diamidino-2-phenylindole; GalC, galactocerebroside C; GFAP, glial fibrillary acidic protein; hNSC, human neural stem cell; IhNSC, immortalized human neural stem cell; MAP2, microtubular-associated protein 2.