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. 2012 Mar 21;1(4):322–332. doi: 10.5966/sctm.2011-0035

Figure 1.

Figure 1.

Effect of hypoxia on in vitro growth of glioblastoma stem-like cells. (A): Phase contrast microscopic images of GBM4 spheres cultured in EF20 medium in normoxia (left panel) and in hypoxia (right panel). Both are ×10 magnification. Scale bar = 100 μm. (B): Sphere diameter of GBM4 and GBM8 cells cultured at clonogenic density in normoxia (white bars) or hypoxia (black bars). (C): Sphere forming assay with GBM4, GBM6, and GBM8 cells plated at 10, 100, or 1,000 cells per milliliter and cultured in normoxia (white bars) or hypoxia (black bars). Spheres were measured 12 days after plating GBM4 and 16 days after plating GBM6 and GBM8 for both normoxic and hypoxic cells. (D): Dissociated GBM4 cells were plated at clonal density and cultured in normoxia and hypoxia, and viable cells were counted 12 days later. (E): Dissociated GBM4 (left panel) and GBM8 (right panel) cells were plated at nonclonal density, and viable cells were counted on days 3 and 6 of culture. The error bars represent standard deviations. ∗, p < .05; ∗∗, p < .01. Abbreviation: GBM, glioblastoma.