Figure 4.

G47Δ kills glioblastoma stem-like cells (GSCs) and suppresses GSC sphere formation in hypoxia. (A): G47Δ mediates killing of normoxic (white bars) and hypoxic (black bars) GBM4 (left panel) and GBM8 (right panel) cells. The data from each infected condition were normalized to those of the corresponding mock control. (B): Sphere forming assay (100 cells per milliliter) performed in normoxia and hypoxia with GBM4 (left panel) and GBM8 (right panel) cells uninfected (mock; white bars) or infected with G47Δ (MOI = 0.2; black bars). Sphere numbers were counted 12 days after plating GBM4 and 16 days after plating GBM8 in both normoxic and hypoxic samples. The data of normoxic-infected, hypoxic-mock, and hypoxic-infected conditions were normalized to the normoxic-mock condition, which was considered 100%. There was no significant difference in virus-mediated reductions between normoxia and hypoxia. The error bars represent standard deviations. Δ, reduction, indicating the effect of G47Δ normalized to mock for both normoxia and hypoxia. ∗, p < .05; ∗∗, p < .01. Abbreviations: GBM, glioblastoma; H, hypoxia; MOI, multiplicity of infection; N, normoxia.