Skip to main content
. 2012 May 8;1(5):396–402. doi: 10.5966/sctm.2011-0061

Figure 3.

Figure 3.

Phenotypic analysis of SOD1-overexpressing human ESC (hESC)-derived spinal motor neurons. (A): Morphologically unhealthy cells were observed in spinal motor neurons (sMNs) derived from G93A SOD1-overexpressing hESCs, whereas WT SOD1 hESC-derived sMNs showed normal morphology. (B): Immunocytochemical staining of cells treated with or without sMN inducers (ATRA and SAG). Both TUNEL (green) and HB9 (red)-positive sMNs were observed in ATRA/SAG-treated G93A SOD1-overexpressing hESCs. (C): Total TUNEL-positive cell ratio. (D): HB9/TUNEL double-positive cell ratio. (E): GFAP/TUNEL double-positive cell ratio. These data indicated sMN-specific cell death in G93A SOD1 hESC-derived sMNs. Approximately 900 cells were used for calculating the ratio of immunopositive cells (TUNEL, HB9, and GFAP). Mean ± SEM; n = 9; Steel-Dwass test; ∗∗, p < .01. Abbreviations: ATRA, all-trans retinoic acid; DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; K1, KhES-1; SOD, superoxide dismutase; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; WT, wild-type.