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. 2012 May 8;1(5):396–402. doi: 10.5966/sctm.2011-0061

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Figure 5. — Ubiquitin staining of SOD1 human ESC-derived sMNs. (A): Immunocytochemical staining of ubiquitin (green) and HB9 (red). An abnormal staining pattern was observed in G93A SOD1-expressing sMNs. Arrowheads show rough staining of ubiquitin or HB9, which might be abnormal ubiquitin inclusions. (B): Quantitative analysis of cells with ubiquitin inclusions (green bars), HB9-positive sMNs (purple bars), and sMNs with ubiquitin inclusions (yellow bars). Mean ± SEM; n = 9; Steel-Dwass test; ∗∗, p < .01. Abbreviations: sMN, spinal motor neuron; SOD, superoxide dismutase; Ub, ubiquitin; WT, wild-type.