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. 2012 May 14;1(5):430–437. doi: 10.5966/sctm.2011-0038

Figure 1.

Figure 1.

Efficiency of differentiation of mouse iPS cells into cardiomyocytes. (A): Number of EBs with beating foci. *, p < .05. (B): Image of an EB derived from iPS cells obtained on day 5. DsRed was expressed in the EB because the mouse iPS cell line 256H18 was used. Scale bars = 300 μm. (C–E): Gene expression in iPS cell-derived cardiomyocytes. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses of the expression levels of undifferentiated cell marker genes (Nanog, Oct3/4, Fgf4, Esg1, Dppa2, and Dppa4) (C) and cardiac marker genes (cardiac actin [CA], Troponin I [TnI], Nkx2.5, GATA4, and Mef2C) (D) in the no-glucose DMEM-treated group and the no-glucose DMEM-untreated group (n = 5 per group). *, p < .05. (E): RT-PCR analyses of cardiac marker genes, such as those for structural proteins (cardiac actin to MLC2v), transcription factors (Nkx2.5 to TEF1), adrenergic receptors (α1A to β2), muscarinic receptors (M2), other proteins (connexin 43 to integrin α7), and markers for undifferentiated cells (Oct3/4 to Fgf4) in native cardiomyocytes, native hepatocytes, iPS cells, and iPS cell-derived cardiomyocytes. Abbreviations: DMEM, Dulbecco's modified Eagle's medium; DsRed, Discosoma red fluorescent protein; iPS, induced pluripotent stem.