Figure 1.

Following 6 months of cryopreservation, human adult olfactory neural progenitors retained their viability and expressed their neural progenitor nature. They formed neurospheres that detached from the substratum and became free-floating; occasional cells expressing a more neuronal-like phenotype could be seen. (A): Merged confocal image demonstrating the presence of the neural stem cell marker-filament nestin (green); the neural crest intermediate filament peripherin (red); and 4′,6-diamidino-2-phenylindole, which highlights nuclear DNA (blue). (B): Nestin only. (C): Peripherin only (red). (D): Cellular integrity is evident with this image.