Figure 4.

Immunolocalization of engrafted hONPs in the 6-hydroxydopamine-lesioned striatum. IPN-transfected hONPs (A–D) and the pretransfected hONPs (E–G) were intact and TH-positive 24 weeks after the engraftment. High-magnification microscopy revealed that the transfected and nontransfected hONPs had TH-positive processes (C, G), which were observed beyond the injection sites (D, F). (H): Pretransfected hONPs; lower power image equivalent to (D) demonstrating similar migration from the site of engraftment as in (D) for the transfected cells. Note that not all of the TH-positive cells double-labeled with human nucleus antigen, reflecting the multifaceted action of hONPs (cell rescue and endogenous stem cell differentiation). Scale bars = 20.0 μm (B, C, G), 50.0 μm (A, E, F), 100.0 μm (H), and 200.0 μm (D). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; hONP, human adult olfactory neural progenitor; IPN, pIRES-Pitx3-Nurr1; TH, tyrosine hydroxylase.