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. 2012 Jul 6;1(7):548–556. doi: 10.5966/sctm.2011-0063

Figure 1.

Figure 1.

NSC depletion in the subventricular zone (SVZ) of Atm−/− mice. (A): Paraformaldehyde-fixed frozen sections of SVZ tissue of adult (postnatal day 90 [P90]) Atm+/+ and Atm−/− mice were stained with antibodies against nestin (red) to identify NSCs and against Ki67 (green) to detect proliferating cells in the SVZ. Cells were counterstained by DAPI (blue), which identifies the nuclei of the NSCs. Scale bars = 50 μm. (B): Total RNA was purified from SVZ tissue of adult (P90) Atm+/+ and Atm−/− mice and subjected to quantitative reverse transcription-polymerase chain reaction analysis for nestin and Ki-67 mRNA expression. Probing for gapdh was used as an internal control. (C): After injection of adult (P90) Atm+/+ and Atm−/− mice with BrdU for a period of 5 days, SVZ sections were stained with an antibody against BrdU to label proliferating cells. Scale bars = 100 μm. BrdU-positive cells in the SVZ of adult (P90) Atm+/+ and Atm−/− mice were counted. Values represent BrdU-positive cell numbers in an SVZ section of Atm+/+ mice ± SD (three independent countings of three sections). Abbreviations: BrdU, bromodeoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole.