Figure 7.

Representative macroscopic and histological images of regenerated hair follicles via transplantation of murine dermal papilla cells (mDPCs) pretreated with 1α,25-dihydroxyvitamin D₃ (VD₃) for 0, 24, or 72 hours. Left column shows representative macroscopic views 8 weeks after transplantation of an mDPC sheet fragment pretreated with VD₃ for 0 (top), 24 (center), and 72 (bottom) hours. Arrows indicate hair regrowth on the skin. Middle and right columns show histological serial sections stained with hematoxylin/eosin (middle) or Hoechst 33342 (right). Two representative samples for each group are shown. Arrowheads indicate DiI-labeled grafted mDPCs. Scale bars = 500 μm (yellow), 100 μm (white). Abbreviations: DP, dermal papilla; HS, hair shaft; SG, sebaceous gland.