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. 2012 Aug 6;1(8):592–603. doi: 10.5966/sctm.2012-0003

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©AlphaMed Press 1066-5099/2012/$20.00/0

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Figure 5. — Drug sensitivity of colorectal cancer cell line-derived putative cancer stem cell (CSC) populations. CD133+, CD166+CD44+, and CD24+CD44+ or their negative counterparts were sorted from the indicated cell lines and cultured in the presence of the indicated concentrations of 5-fluorouracil, oxaliplatin, or irinotecan. After 72 hours, cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) staining. Percentages (averages ± SD of quadruplicate cultures) of surviving cells are reported. Statistical evaluation was performed by two-tailed Student's t test. Only significantly increased percentages of survival (p ≤ .05) of putative CSCs relative to negative counterparts or parental cells are indicated. Reported data refer to one representative experiment out of two performed with similar results. Abbreviation: Conc, concentration.