Figure 1.

Characterization of induced pluripotent stem cell (iPSC) lines: representative pictures. (A): Polymerase chain reaction analysis of endogenous pluripotency markers SOX2 (151 bp), KLF4 (397 bp; lower band corresponds to primer dimers), OCT4 (144 bp), and GAPDH (94 bp). (B): Phase image and SSEA4/DAPI staining of iPSC colony. Magnification, ×40. (C): Normal karyotype. (D): Hematoxylin and eosin staining of a teratoma showing differentiation into ectoderm, mesoderm, and endoderm. (E): Flow cytometry analysis for pluripotency markers OCT4, SOX2, NANOG, SSEA4, and TRA1-60. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SSEA4, stage-specific embryonic antigen-4.