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Flow cytometry analysis of SSEA4 protein expression. SSEA4 protein levels were quantified at three different passages in three SSEA4-low induced pluripotent stem cell (iPSC) lines and one representative SSEA4-high iPSC line. The percentages of cells expressing the marker (gray) above the isotype-control cells (clear) are shown in the graph. *, Cells sorted for transcriptome analysis. Abbreviation: SSEA4, stage-specific embryonic antigen-4.
