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. 2012 Sep 5;1(9):673–684. doi: 10.5966/sctm.2012-0053

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©AlphaMed Press 1066-5099/2012/$20.00/0

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Figure 1. — Yield, viability, and frequency of human PSCs in human white adipose tissue. (A): Total SVF cell yield, as represented by total SVF cells (10⁶) per 100 ml of tissue lipoaspirate. Blood and saline of lipoaspirate fluid are not included in these calculations. Each bar indicates the number of lipoaspirate samples falling within the stated range of values. (B): Percentage of cell viability, as determined by percentage of DAPI− cells by flow cytometry. (C): Percentage of hematopoietic cells, as determined by percentage of CD45+ cells among viable (DAPI−) cells by flow cytometry. (D): Percentage of pericytes, as determined by percentage of CD146+, CD34− cells among viable (DAPI−) cells. (E): Percentage of adventitial cells, as determined by percentage of CD146−, CD34+ cells among viable (DAPI−) cells. (F): Percentage of human PSCs, as determined by percentage of pericytes + percentage of adventitial cells. Abbreviations: PSC, perivascular stem cell; SVF, stromal vascular fraction.